ENZYME PREDICTOR

ENZYME PATTERNS

The following enzymes and cleavage patterns are the ones used by Enzyme-Predictor.


Enzyme nameP4P3P2P1P1'P2'
Arg-C proteinase---R--
Asp-N endopeptidase----D-
Caspase 1 F, W, Y, or L -H, A or TDnot P, E, D, Q, K or R -
Caspase 2DVADnot P, E, D, Q, K or R-
Caspase 3DMQDnot P, E, D, Q, K or R-
Caspase 4LEVDnot P, E, D, Q, K or R-
Caspase 5 L or W EHD--
Caspase 6VE H or I Dnot P, E, D, Q, K or R-
Caspase 7DEVDnot P, E, D, Q, K or R-
Caspase 8 I or L ETDnot P, E, D, Q, K or R-
Caspase 9LEHD--
Caspase 10IEAD--
Chymotrypsin-high specificity (C-term to [FYW], not before P)--- F or Y not P-
---W not M or P -
Chymotrypsin-low specificity (C-term to [FYWML], not before P)--- F,L or Y not P-
---W not M or P -
---M not P or Y -
---H not D,M,P or W -
EnterokinaseD or ND or ND or NK--
Factor Xa A,F,G,I,L,T,V or M D or E GR--
Formic acid---D--
Glutamyl endopeptidase---E--
GranzymeBIEPD--
Hydroxylamine---NG-
Iodosobenzoic acid---W--
LysC---K--
NTCB (2-nitro-5-thiocyanobenzoic acid)----C-
Pepsin (pH1.3)-not H,K, or Rnot Pnot RF,L,W or Ynot P
-not H,K, or Rnot PF,L,W or Y-not P
Pepsin (pH>2)-not H,K or Rnot Pnot RF or Lnot P
-not H,K or Rnot PF or L-not P
Proline-endopeptidase-- H,K or R Pnot P-
Staphylococcal peptidase I--not EE--
Thrombin--GRG-
A,F,G,I,L,T,V or M A,F,G,I,L,T,V,W or A PR not D or E not DE
Trypsin (please note the exceptions!)--- K or R not P-
--WKP-
--MRP-
Modified-chymotrypsin---F,W,Y or Lnot P-
elastase---A,V,I,L,G or RG,P,A,L or F-
Cyanogen-bromide---M--
Proline-endopeptidase_diff---P--
Plasmin---K or R-
CathepsinD---A,V,L,I,P,M,F or WA,V,L,I,P,M or F-
Enzyme nameP4P3P2P1P1'P2'


The exception rules:


The above cleavage rules do not apply, i.e. no cleavage occurs, with the following compositions of the cleavage sites:
Enzyme nameP4P3P2P1P1'P2'
Trypsin-- C or D KD-
--CKH or Y-
--CRK-
--RRH or R-